Proteomics
De Novo Protein Sequencing Technology

Our commitment to pursue the most pioneering, leading-edge proteomic technologies has driven us to add de novo Protein and Antibody Sequencing to our service provision. “This year we will focus on ensuring a seamless results-driven service and apply our extensive knowledge and experience across a broad range of pioneering proteomic disciplines to provide our customers with […]

Proteomics
Proteomics: A Timeline

Proteomics is the analysis of the entire protein complement of a cell, tissue, organism, or species. The application of technologies to identify and quantify proteins is crucial in early disease diagnosis, prognosis, and drug development. Proteomics as a discipline has its roots in the late 1800s with the emergence of rudimentary mass spectrometry. The following […]

Proteomics
Our Story – From formation to specialisation in proteomics

Hi, I’m Jan and I’m new around here. In fact, I’m new science altogether, but I am definitely learning new things every day and enjoying being a part of such a dynamic community. I wanted to learn a bit more about the history of DC Biosciences, how it became so pioneering in the field of […]

Jan Hewitt
Proteomics
EASI-tag, and why it is really exciting

There was an interesting paper recently:     A new type of Isobaric Labelling reagents? Do we really need more? Isn’t this what TMT and iTRAQ already do? Why is this important?   Isobaric Labelling In Isobaric Labelling, each sample is modified with different versions of a chemical label which are near identical but have […]

Dr. Armel Nicolas
Proteomics
Where to draw the line? Hypothesis Testing in Proteomics

So, you’ve got this beautiful proteomics experiment done. You just got your data (yay!), let’s say ratios of treated vs control, 3 replicates, simple experiment. But that’s thousands of protein groups, most of which are probably not interesting at all. We want to find out which proteins are regulated. How do we do it?   […]

Dr. Armel Nicolas
Proteomics
ADAPTED Project meeting in Edinburgh – understanding APOE

This week Francesco Rao attended a progress meeting for the ADAPTED project. For this meet up the city of choice was Edinburgh, some might argue the most beautiful city in the world! After a successful launch year, this was the first consortium meeting in 2018. With someone in the world developing Dementia every 3 seconds, the […]

Sarah Macdonald
Proteomics
Through the Looking Glass: Peptide identification

In previous instalments, we looked at what happens to the poor peptides we send to their doom down the rabbit hole and into the strange wonderland that is the inside of a mass spectrometer[1]. Now, it’s all fine and good to fragment peptides into tiny little pieces, I for one am all for it. I […]

Armel Nicolas
Proteomics
Follow The White Rabbit – Interpreting Individual Peaks – MS2 spectra

Hi everyone, and welcome to a glorious New Year! 2017 was fun, wasn’t it? I for one so much look forward to 2018 being slightly more normal. Oh what the hell, who am I kidding, I’ll get some popcorn already. Today, we will be talking about MS2 spectra, that is, the spectra resulting from the […]

Armel Nicolas
Proteomics
Down The Rabbit Hole: what does a peptide look like in MS?

Interpreting Individual Peaks – part 1 If you have ever looked at a mass-spectrometer output file, you may have wondered how it is possible to make sense of all of these peaks. I mean, there really is a lot of them! Here, we will talk a bit about the basic rules which govern the behaviour […]

Armel Nicolas
Proteomics
What a tangled web we unweave – deciphering raw proteomics data

Today we will be speaking of proteomics data files and their contents. We will also talk a bit about proteomics file formats, and why they are a mess. In a typical case, you would have been running peptide samples on a mass-spectrometer using a gradient to separate peptides by some physicochemical property so they don’t […]

Armel Nicolas
Point of View