This week Francesco Rao attended a progress meeting for the ADAPTED project. For this meet up the city of choice was Edinburgh, some might argue the most beautiful city in the world! After a successful launch year, this was the first consortium meeting in 2018. With someone in the world developing Dementia every 3 seconds, the […]
In previous instalments, we looked at what happens to the poor peptides we send to their doom down the rabbit hole and into the strange wonderland that is the inside of a mass spectrometer[1]. Now, it’s all fine and good to fragment peptides into tiny little pieces, I for one am all for it. I […]
Hi everyone, and welcome to a glorious New Year! 2017 was fun, wasn’t it? I for one so much look forward to 2018 being slightly more normal. Oh what the hell, who am I kidding, I’ll get some popcorn already. Today, we will be talking about MS2 spectra, that is, the spectra resulting from the […]
Interpreting Individual Peaks – part 1 If you have ever looked at a mass-spectrometer output file, you may have wondered how it is possible to make sense of all of these peaks. I mean, there really is a lot of them! Here, we will talk a bit about the basic rules which govern the behaviour […]
Today we will be speaking of proteomics data files and their contents. We will also talk a bit about proteomics file formats, and why they are a mess. In a typical case, you would have been running peptide samples on a mass-spectrometer using a gradient to separate peptides by some physicochemical property so they don’t […]
From lots of gibberish to useful data… This post is destined to become the introduction of a new series of glorious blog entries on the subject of LC-MS/MS data analysis. In this series we would like to talk about proteomics data analysis and the different steps it involves. The series will focus on the […]
Last week, my colleague Francesco Rao (DC Biosciences’ CSO) and I travelled to Bonn to participate in a progress meeting for ADAPTED (Alzheimer’s Disease Apolipoprotein Pathology for Treatment Elucidation and Development). Bonn, the birthplace of Beethoven (though everyone’s favourite tortured pre-romantic composer spent most of his years in Vienna), is a relatively small city […]
Mass spectrometry-based proteomics is an amazing and still fast evolving field. The way clever people constantly come up with new ways to tweak experiment design to tackle wholly different biological questions never ceases to amaze me. When I started in MS, I had mostly heard of people using MALDI-TOF instruments. Orbitraps were just emerging as […]
Thermo Fisher releases TMT 11-plex. Thermo Fisher recently released an additional channel for TMT, which means you can now combine up to 11 different samples as one for better sample comparability and more complete data! It is now possible to use “isotopologue” encoding to split the 131 (last) channel into 131-N and 131-C to get […]
New project to explore overlooked area of Alzheimer’s Disease research Alzheimer’s disease (AD) affects over 35 million people world-wide at present, these numbers are expected to grow substantially over the next few decades and there are currently no medicines which slow the progress of the disease. To boost the development of new medicines for AD, […]
