Proteomics is the analysis of the entire protein complement of a cell, tissue, organism, or species. The application of technologies to identify and quantify proteins is crucial in early disease diagnosis, prognosis, and drug development. Proteomics as a discipline has its roots in the late 1800s with the emergence of rudimentary mass spectrometry. The following timeline is designed to show the development from these primitive origins to today’s sophisticated applications and practice.
DC Biosciences is proud to offer our expertise in innovative proteomics and is dedicated to finding solutions for research in all fields of drug discovery and protein analysis. Our small but highly-specialised team sets the standard for reliable, cost-effective, and high-quality solutions for researchers.
Being both knowledgeable and accomplished in the field, our team will design, perform, and document your experiment creating the best possible workflow for your project. We offer a friendly and flexible approach with open communication and support at every stage.
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- George Paget Thomson. J.J Thomson A Biography. Encyclopædia Britannica. 2020 December 14; https://www.britannica.com/biography/J-J-Thomson
- Prof. K. Biemann et al. Structure of an Odd Nucleoside from Serine‐Specific Transfer Ribonucleic Acid. Wiley Online Library. 1966 June; https://doi.org/10.1002/anie.196605902
- O’Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007-21. PMID: 236308; PMCID: PMC2874754.
- Anderson NG, Anderson NL. The Human Protein Index. JAMA. 1981;246(22):2620–2621. doi:10.1001/jama.1981.03320220070033
- Matthias S. Wilm, Matthias Mann. Electrospray and Taylor-Cone theory, Dole’s beam of macromolecules at last? International Journal of Mass Spectrometry and Ion Processes, 1994 September 22; Volume 136, Issues 2–3.
- Wilkins, M.R., Sanchez, J.-C., Gooley, A.A., Appel, R.D., Humphery-Smith, I., Hochstrasser, D.F., et al. .; Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it; Biotechnology and Genetic Engineering Reviews, (1996); 13(1): 19–50.
Richard M. Caprioli, Terry B. Farmer, and Jocelyn Gile. Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS. 1997; Analytical Chemistry 69 (23), 4751-4760 DOI: 10.1021/ac970888i
- Gygi, S., Rist, B., Gerber, S. et al. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat Biotechnol 17, 994–999 (1999). https://doi.org/10.1038/13690
Alexander Makarov. Electrostatic Axially Harmonic Orbital Trapping: A High-Performance Technique of Mass Analysis. Analytical Chemistry 2000 72 (6), 1156-1162
Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics
- Ross et al. Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging Reagents. Molecular & Cellular Proteomics. 2004 September 22;
- Wilhelm, M., Schlegl, J., Hahne, H. et al. Mass-spectrometry-based draft of the human proteome.2014 May 28; Nature 509,582–587
- Kim, MS., Pinto, S., Getnet, D. et al. A draft map of the human proteome. Nature 2014 May 28; 509, 575–581
- Swaminathan, J., Boulgakov, A., Hernandez, E. et al. Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures. Nat Biotechnol 36, 1076–1082 (2018).
- Evelina Sjöstedt et al. An atlas of the protein-coding genes in the human, pig, and mouse brain. Science 06 Mar 2020; Vol. 367, Issue 6482, eaay5947