Click-&-Go Protein Enrichment Kit *for capture of azido-modified proteins*
Protein Enrichment Kit for covalent capture of azido-tagged proteins.
Click-&-Go™ Protein Enrichment Kit is an efficient, biotin-streptavidin free tool for capture of azido-modified proteins on an alkyne-agarose resin. The azide modification can occur via metabolic feeding, enzymatic addition, or chemical modification. The azido-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the alkyne-agarose resin supplied. Once covalently attached to the resin via copper catalysed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon protease digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis.
The biotin-free enrichment improves signal to noise by eliminating non-specific binding and increasing selectivity, thus improving detection of low abundant proteins. This approach is fully compatible with widely used MS techniques including iTRAQ and ICAT.
The biotin-free, covalent capture approach is superior to biotin or lectin based enrichment approaches, and ideal for proteomics, biomarker discovery, posttranslational modification (PTM) analysis, and more.
The Click-&-Go™ Protein Enrichment Kit can be used as direct replacement of Click-iT® Protein Enrichment Kit
Biotin-free covalent capture