A water-soluble, azide-reactive fluorescent probe.
Cy®3 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalysts or elevated temperatures. In applications where the presence of copper is a concern, Cy3 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532nm or 555nm laser line and visualised with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.
Cy®3 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilised cells due to high backgrounds.
This is sulfonated dye is also known as sulfo-Cyanine3. Cy® Dye is a trademark of GE Healthcare.
532, 555 or 568nm
532 or 555nm
Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™ 549
Water, DMF, DMSO