Cy3 Methyltetrazine

Methyltetrazine-activated Cy3 dye that reacts with TCO-containing compounds via an Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require a Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bio-orthogonal reaction that possesses exceptional kinetics (k > 800 -1 s-1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bio-orthogonal reaction pair described to date.

Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualised with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

Cy®Dye is a trademark of GE Healthcare.