An azide-activated, water-soluble near-IR probe.
The Cy7 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclo-octyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Cy7 is a far-red (near-infrared) emitting dye with a peak excitation at 753 nm and maximum emission at 776 nm in PBS. Cy7 is spectrally almost identical to other near-IR dyes, including of Alexa Fluor® 750, DyLight® 750, or IRDye™ 750.
Alexa Fluor® 750, RDye™ 750RD, CF™ 750 Dye, DyLight® 750
Water, DMF, DMSO