An alkyne-activated, cleavable agarose for enrichment of azide-tagged bio-molecules.
Dde Agarose Alkyne is a 6% cross-linked agarose resin that is activated with alkyne groups through a cleavable Dde linker. Once bio-molecules are covalently captured to the resin, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins. Treatment with 2% hydrazine yields a highly enriched population of proteins. This product is adequate to work in batch or column purifications (Low Pressure).
Batch or column purifications
5-20µmol azide groups per ml resin