An alkyne-reactive, cleavable biotin probe that allows for elution of the captured bio-molecules with hydrazine.
Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. Dde biotin probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety linked to an azide moiety through a spacer arm containing a Dde linker. Captured bio-molecules can be efficiently released, typically >90%.
DMF, DMSO, THF, DCM, Chloroform