PC Biotin-PEG3-NHS Ester

PC Biotin-NHS Ester as a unique amine reactive, photo-cleavable biotin reagent that allows for reagent-free release of the captured bio-molecules from streptavidin. This reagent contains a biotin moiety linked to the amine reactive N-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photo-cleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured bio-molecules can be efficiently photo-released, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm²).