TAMRA-Azide-Desthiobiotin
An analogue of TAMRA-Azide-Biotin tri-functional reagent that allows for easy elution from streptavidin.
| 10mg | £234.00 | |
| 25mg | £714.00 |
Desthiobiotin is a single-ring, sulfur-free analogue of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimise co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
1145.33
C57H82N11O14
N/A
DMF, DMSO, DCM, THF, Chloroform
>95%
Red amorphouse solid to red oil
-20°C
Room Temperature
