Affinity pull-downs are one of the most common types of proteomic experiments performed in any research laboratory. Combining such experiments with mass spectrometry can be challenging, but it is a very powerful approach when studying protein-protein interactions.
Understanding protein-protein interactions in cellular pathways is essential for comprehending biological processes. Mass spectrometry (MS)-based proteomics is a powerful tool to analyse interactomes. Unlike traditional western blot (WB) analysis, MS can identify all the enriched proteins in a completely unbiased manner.
DC Biosciences offer a range of pull-down proteomic experiments, Our experts will guide you from experimental design through to completion and assist you in avoiding the many pitfalls associated with mass spectrometry analysis of affinity-based experiments.