Need to quickly determine the molecular mass of your protein? Assess if your sample has protein contaminants? Intact protein mass measurement is the answer.
The majority of mass spectrometry (MS)-based proteomic experiments performed today follow the “bottom-up” principle. That is, proteins are digested into peptides and analysed by LC-MSMS. The data obtained from the peptides is then used to re-create information at the protein level.
It is possible to analyse intact proteins in mixtures of low-complexity. This can be a convenient and rapid means of assessing the purity of a protein and/or determining if the protein in the sample has the expected molecular mass (Mr).
DC Biosciences offers intact mass determination of your protein. Simply provide us with the sample and information regarding the composition of the buffer, and we will do the rest.